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The location on the DHFR gene is shown in parenthesis in the main column. Expression values corresponds to your mobile strain inside the column heading.
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(2020) Altered expression of an outstanding Handle protease in E. coli reshapes the in vivo mutational landscape of the design enzyme
Protein mutational landscapes are formed because of the mobile surroundings, but vital variables as well as their quantitative consequences are often unknown. in this article we display that Lon, a top quality control protease naturally absent in widespread E. coli expression strains, substantially reshapes the mutational landscape of your metabolic enzyme dihydrofolate reductase (DHFR). choice beneath problems that resolve very Lively check here mutants reveals that 23.
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